human small cell lung carcinoma line nci h1930  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas

doi: 10.1016/j.bbrep.2026.102587

Figure Lengend Snippet: Immunohistochemical analysis of SLC35D3 expression in human tumors, normal tissues, and positive-control samples. (A) SLC35D3 staining in human tumors: (a) primary colon tumor; (b) corresponding lymph-node metastasis; (c) normal adjacent tissue (NAT) of a; (d) primary rectal tumor; (e) corresponding lymph-node metastasis; (f) NAT of d; (g–h) small-cell lung carcinoma (SCLC); (i) pancreatic neuroendocrine neoplasm; (j) pancreatic islet tumor. (B) SLC35D3 staining in human normal tissues: (a) cerebrum; (b) bone marrow; (c) lung; (d) heart; (e) liver; (f) kidney; (g) eye; (h) colon; (i) adrenal gland; (j) pancreas; (k) hypophysis; (l) stomach; (m) small intestine; (n) prostate. Arrowheads indicate SLC35D3-positive cells. (C) SLC35D3 staining of negative and positive control cell lines: (a) HCT 116-mock (negative control); (b) HCT 116-hSLC35D3 (engineered overexpression); (c) NCI–H1930 (endogenously SLC35D3-expressing).

Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL-5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL-229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL-5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.

Techniques: Immunohistochemical staining, Expressing, Positive Control, Staining, Negative Control, Over Expression

Journal: Biochemistry and Biophysics Reports

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas

doi: 10.1016/j.bbrep.2026.102587

Figure Lengend Snippet: Validation of cell-surface SLC35D3 expression in cancer cell lines by flow cytometry and comparison with CCLE transcriptomic data. Flow cytometry histograms of cell-surface SLC35D3 staining in human cancer cell lines (HCT 116, LoVo, QGP-1, NCI–H1930, and SNU-16). For each cell line, the MFI ratio (anti-SLC35D3/isotype) and the corresponding mRNA expression level (log2[TPM+1]) from the CCLE are indicated below the histogram. HCT 116 served as a negative control and showed minimal surface staining, consistent with a CCLE value of log2[TPM+1] = 0.0.

Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL-5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL-229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL-5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.

Techniques: Biomarker Discovery, Expressing, Flow Cytometry, Comparison, Staining, Negative Control

Journal: Materials Today Bio

Article Title: CRISPR-engineered human lung organoids with a biomolecular condensate reporter enable mechanistic toxicity monitoring

doi: 10.1016/j.mtbio.2026.102972

Figure Lengend Snippet: Dose–response profiling of representative toxicant groups in A549 and G3BP1–mCherry hLO. Cell viability was measured after 24 h exposure to five representative toxicants in two in vitro lung models: A549 (2D monolayer) and G3BP1–mCherry knock-in human lung organoids (hLOs). (A) Dose–response curves for the disinfectant/biocide group— didecyldimethylammonium chloride (DDAC), 2-octyl-4-isothiazolin-3-one (OIT), and polyhexamethylene guanidine (PHMG-p). (B) Dose–response curves for industrial chemicals—4,4′-MDI and acrylonitrile. Curves were fitted using nonlinear regression (log[inhibitor] vs . normalized response, variable-slope model) in GraphPad Prism. Data are presented as mean ± SD. (C) Heatmap displaying relative log 2 (IC 50 ) values comparing A549 and hLO models. Each IC 50 value was normalized to the mean IC 50 of the respective compound. The color scale represents deviations from the mean, with blue indicating higher sensitivity and red indicating lower sensitivity. The scale ranges from −2 to +2, corresponding to up to a fourfold deviation from the mean IC 50 ; values outside this range were clipped for clarity.

Article Snippet: A549 human lung epithelial carcinoma cells, purchased from the American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's modified eagle medium, high glucose (LM001-05, WELGENE, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (16000044, Gibco, Waltham, MA, USA), 1% penicillin/streptomycin (15040122, Gibco), 1 mM sodium pyruvate (11360070, Gibco), and 1 × GlutaMAX (35050061, Gibco).

Techniques: In Vitro, Knock-In

Journal: Materials Today Bio

Article Title: CRISPR-engineered human lung organoids with a biomolecular condensate reporter enable mechanistic toxicity monitoring

doi: 10.1016/j.mtbio.2026.102972

Figure Lengend Snippet: Comparative toxicant sensitivity and stress granule responses in G3BP1–mCherry hLOs and conventional lung models. (A) Cell viability analysis of sodium arsenite (NaAsO 2 ), benzalkonium chloride (BAC), and 1,2-benzisothiazolin-3-one (BIT) was evaluated in three lung models: A549 (3D spheroid), patient-derived lung cancer organoid (LCO), and G3BP1–mCherry knock-in lung organoid (hLO). IC 50 values were determined by nonlinear regression (log[inhibitor] vs . normalized response, variable-slope model) using GraphPad Prism. Data are presented as mean ± SD. (B) Heatmap depicting relative log 2 (IC 50 ) values to illustrate model-specific differences in toxicant sensitivity. Each IC 50 value was normalized to the mean IC 50 of the respective compound. Blue and red represent higher and lower sensitivity relative to the mean, respectively. (C) Immunofluorescence staining showing stress granule (SG) formation in patient-derived LCOs and G3BP1–mCherry hLOs after 1 h exposure to sodium arsenite (0, 50, or 100 μM). G3BP1 (green) indicates SG localization, and nuclei were counterstained with Hoechst 33342 (blue). Representative images at intermediate concentrations (12.5, 25, 75 μM) are shown in . Scale bars, 20 μm. (D) Quantification of SG numbers per cell in patient-derived LCOs and hLOs following sodium arsenite treatment (0–100 μM). Bars represent mean ± SD. Statistical analysis was performed using two-way ANOVA followed by Sidak's multiple comparisons test (ns, not significant; *** p < 0.001; **** p < 0.0001).

Article Snippet: A549 human lung epithelial carcinoma cells, purchased from the American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's modified eagle medium, high glucose (LM001-05, WELGENE, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (16000044, Gibco, Waltham, MA, USA), 1% penicillin/streptomycin (15040122, Gibco), 1 mM sodium pyruvate (11360070, Gibco), and 1 × GlutaMAX (35050061, Gibco).

Techniques: Derivative Assay, Knock-In, Immunofluorescence, Staining